Indoor air quality (IAQ) and thermal comfort are vital to occupant health, productivity, and overall well-being. Conventional methods to improve these conditions—such as installing or upgrading mechanical ventilation systems—can be costly, energy-intensive, and disruptive to regular operations. This study explores whether strategic furniture arrangement can offer a passive, low-cost solution to enhance indoor air distribution and thermal comfort in a university meeting room. A mixed-method approach was employed, integrating empirical environmental monitoring with Computational Fluid Dynamics (CFD) simulations. Baseline data, including temperature, humidity, CO₂, PM₂.₅, and PM₁₀, were collected continuously over two weeks in an unventilated condition to establish the existing IAQ profile. These data were then used to calibrate CFD models simulating two furniture layout scenarios under fully occupied, air-conditioned conditions. The empirical findings revealed minimal vertical stratification but indicated thermal discomfort caused by insufficient air circulation. CFD results showed that while both layouts maintained acceptable temperatures (20–22 °C), the original configuration created stagnant zones with airflow velocities below 0.2 m/s. In contrast, shifting the furniture by 1.20 meters significantly improved airflow velocity (average 0.285 m/s) in the occupants’ breathing zone and reduced stagnant areas. The study concludes that modest, strategic adjustments in spatial configuration can meaningfully improve IAQ and thermal comfort without requiring mechanical intervention. These results offer practical, evidence-based recommendations for architects and facility managers seeking to enhance indoor environmental quality through design, particularly in educational and office environments constrained by structural or financial limitations.